Tõrkeid taluv võrk, mis baseerub Linux süsteemil ja kasutab vana riistvara

Networking at enterprise level is fast, reliable,fault-tolerant, expensive and usually includes a vendor lock-in. This thesis tries to take the good qualities of the enterprise level networking and remove the negative side effects. One possible way to solve the problem is to create a networking solution that uses obsolete hardware and free software to neglect the negative properties of enterprise networking. This kind of solution can be achieved without any need for more system administration knowledge or know-how. Moreover, the thesis conducts an analysis of the previous work done by other people on fault tolerance in networking and offers the author’s own solution implementation. The main result of this thesis is a low-cost solution to Internet service provider and router failover. The tests that were carried out show that it is possible to create a network that, even in the case of ISP and/or router failure, can continue functioning at full speed without significant delays and without disrupting the presently occurring data transmission. The thesis’s work uses computer hardware that has been announced old by its previous owners and software that is open source and readily deployable with a little skill. From a theoretical point of view it should be acknowledged that the author understands that software routing is not as good as hardware routing, however the overall idea was to offer a solution which could theoretically perform as similar as possible to the hardware routing

Warfarin-exposed zebrafish embryos resembles human warfarin embryopathy in a dose and developmental-time dependent manner - From molecular mechanisms to environmental concerns

Warfarin is the most worldwide used anticoagulant drug and rodenticide. Since it crosses placental barrier it can induce warfarin embryopathy (WE), a fetal mortality in neonates characterized by skeletal deformities in addition to brain hemorrhages. Although the effects of warfarin exposure in aquatic off target species were already described, the particular molecular toxicological mechanisms during early development are still unclear. Here, we used zebrafish (Danio rerio) to describe and compare the developmental effects of warfarin exposure (0, 15.13, 75.68 and 378.43 mM) on two distinct early developmental phases (embryos and eleuthero-embryos). Although exposure to both developmental phases induced fish mortality, only embryos exposed to the highest warfarin level exhibited features mimicking mammalian WE, e.g. high mortality, higher incidence of hemorrhages and altered skeletal development, among other effects. To gain insights into the toxic mechanisms underlying warfarin exposure, the transcriptome of embryos exposed to warfarin was explored through RNA-Seq and compared to that of control embryos. 766 differentially expressed (564 up- and 202 down-regulated) genes were identified. Gene Ontology analysis revealed particular cellular components (cytoplasm, extracellular matrix, lysosome and vacuole), biological processes (mainly amino acid and lipid metabolism and response to stimulus) and pathways (oxidative stress response and apoptosis signaling pathways) being significantly over-represented in zebrafish embryos upon warfarin exposure. Protein-protein interaction further evidenced an altered redox system, blood coagulation and vasculogenesis, visual phototransduction and collagen formation upon warfarin exposure. The present study not only describes for the first time the WE in zebrafish, it provides new insights for a better risk assessment, and highlights the need for programming the rat eradication actions outside the fish spawning season to avoid an impact on off target fish community. The urge for the development of more species-specific anticoagulants for rodent pest control is also highlighted.Portuguese Foundation for Science and TechnologyPortuguese Foundation for Science and Technology [UID/Multi/04326/2019]project MET2VI - Ministerio de Ciencia, Innovacion y Universidades of the Spanish Government [RTI2018-099029-A-I00]info:eu-repo/semantics/publishedVersio